Two-hybrid system: yeast LexA-BD/VP16-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast lexA-BD/VP16-AD. Full-length, DNA binding domain and C terminal domain
Source was cell extract of Cos cell line; bait produced from transfected construct; prey produced from transfected construct.
pnr-DBD and pnr-CT recognized by the D10 antibody
Source was cell extract of Cos cell line; bait produced from transfected construct; prey produced from transfected construct.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
Source was cell extract of Cos cell line; bait produced from transfected construct; prey produced from transfected construct.
Double immunoprecipitation
