FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
CTCF, SA1
FlyBase ID
FBig0000120877
Interaction Network
Interactions Browser links
CTCF network
SA1 network
Reported Interactions
FBrf0248103-1.PD.MW
Reference
Kaushal et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CTCF 

CTCF

bait

GFP tag

CTCF 
CTCF 
SA1 

SA

prey
SA1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
CTCF 
N-terminal domain
sufficient binding region
aa 1-293

coordinates relative to CTCF-PA

CTCF 
N-terminal domain residues 248,250
mutation decreasing interaction
aa 248,250

coordinates Y248A,F250A, relative to CTCF-PA

SA1 
interaction domain
sufficient binding region
aa 102-1085
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction with CTCF was tested with a cohesin subcomplex consisting of SA and vtd. Captured as two binary interactions.

(Kaushal et al., 2021)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Kaushal et al., 2021)
FBrf0253430-9.PD.MS
Reference
Kaushal et al., 2022
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CTCF 

CTCF

bait

GFP tag

CTCF 
SA1 

SA

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
CTCF 
N terminal domain
sufficient binding region
aa 1-293

coordinates relative to CTCF-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type, nuclear embryonic extract.

(Kaushal et al., 2022)
External Crossreferences and Linkouts ( 0 )
References (2)