Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey produced from an embryonic cDNA library as well as a Drosophila 0-2 h embryonic yeast two-hybrid library).
Two-hybrid system: yeast BD/GAL4-AD.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Source was 0-3 hr embryonic cell lysates; bait produced from endogenous gene; prey produced endogenous gene.
