FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Spt5, Polr2A
FlyBase ID
FBig0000121159
Interaction Network
Interactions Browser links
Spt5 network
Polr2A network
Reported Interactions
FBrf0161558-1.coIP.WB
Reference
Saunders et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Spt5 

Spt5

bait
Polr2A 

RpII215

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Immunoprecipitated Pol II was recognized by antibodies specific for both Ser2 and Ser5 phosphorylated versions.

(Saunders et al., 2003)

Source was unspecified Drosophila cell extract; bait produced from endogenous gene; prey produced from endogenous gene.

(Saunders et al., 2003)
FBrf0161558-4.coIP.WB
Reference
Saunders et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Spt5 

Spt5

bait

Flag tag

Polr2A 

RpII215

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was extracts of an unspecified stable Drosophila cell line; bait produced from transgenic construct; prey produced from endogenous gene.

(Saunders et al., 2003)
External Crossreferences and Linkouts ( 0 )
References (1)