Nup54, Nup58 Physical Interaction report

Collection

Source/Stage

Cell line used

S2-unspecified

Participants

Corresponds to

Reported as

Role

Note

Nup54

bait

3XFlag tag

Nup58

prey

HA tag

Nup58

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Nup58

carboxy-terminal region

sufficient binding region

unspecified

coordinates relative to Nup58-PA

Nup58

N-terminal region

mutation disrupting interaction

unspecified

coordinates relative to Nup58-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Munafò et al., 2021)

FBrf0249027-10.coIP.WB

Reference

Munafò et al., 2021

Description

physical association

Assay

Collection

Source/Stage

Cell line used

proximity-dependent biotin identification

Participants

Corresponds to

Reported as

Role

Note

Nup58

bait

3XFlag tag

Nup54

prey

HA tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

There is a typo in the figuring listing Nup68 instead of 58.

(Munafò et al., 2021)

Source was cell extract of an OSC cell line; bait produced from transfected construct; prey produced from transfected construct.

(Munafò et al., 2021)

FBrf0249027-16.PL.MS

Reference

Munafò et al., 2021

Description

physical association

Assay

Identification by mass spectrometry

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Nup54

bait

BASU (biotin ligase)

Nup58

prey

biotin tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of an OSC line; bait was from transfected construct; prey derived from endogenous gene.

(Munafò et al., 2021)

FBrf0249027-2.coIP.WB

Reference

Munafò et al., 2021

Description

physical association

Assay

proximity-dependent biotin identification

Collection

Source/Stage

Cell line used

S2-unspecified

Participants

Corresponds to

Reported as

Role

Note

Nup58

bait

3XFlag tag

Nup54

prey

HA tag

Nup54

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Nup54

carboxy-terminal region

sufficient binding region

unspecified

coordinates relative to Nup54-PA

Nup54

N-terminal region

mutation disrupting interaction

unspecified

coordinates relative to Nup58-PA

Nup54

Nup54MB

mutation disrupting interaction

unspecified

coordinates relative to Nup58-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Munafò et al., 2021)

FBrf0249027-21.PL.MS

Reference

Munafò et al., 2021

Description

physical association

Assay

Identification by mass spectrometry

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Nup58

bait

BASU (biotin ligase)

Nup54

prey

biotin tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of an OSC line; bait was from transfected construct; prey derived from endogenous gene.

(Munafò et al., 2021)

FBrf0249027-9.coIP.WB

Reference

Munafò et al., 2021

Description

physical association

Assay

Collection

Source/Stage

Cell line used