FB2026_02 , released June 18, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000121518
Interaction Network
Interactions Browser links
Atg8a network
Tak1 network
Reported Interactions
FBrf0252505-3.Y2H
Description
physical association
Assay
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Atg8a

bait

fused to LexA DNA-binding domain

Tak1 

Tak1

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo third instar larva expression library).

FBrf0252505-4.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Atg8a

bait

GST tag

Tak1 

Tak1

prey

His tag

Tak1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
LIR docking site residues 48-49
necessary binding region
aa 48,49

K48A, Y49A.

Tak1 
LIR domain residues 669 and 672
necessary binding region
aa 669, 672

W669A, I672A; coordinates relative to Tak1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

External Crossreferences and Linkouts ( 0 )
References (1)