FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000121784
Interaction Network
Interactions Browser links
SREBP network
Cdk8 network
Reported Interactions
FBrf0255022-1.PD.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

SREBP

bait

GST tag

Cdk8 

Cdk8

prey

His tag

Cdk8 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
interaction domain residues 36-41
necessary binding region
aa 36-41

aa DMLDII36-41AAAAAA; coordinates relative to SREBP-PA

Cdk8 
N-terminal domain
sufficient binding region
aa 1-262

coordinates relative to Cdk8-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

Fig5A-D, Fig6B

FBrf0255022-3.Y2H
Reference
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

SREBP

bait

fused to GAL4 DNA-binding domain

Cdk8 

Cdk8

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
N-terminal domain
sufficient binding region
aa 1-50

coordinates relative to SREBP-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

External Crossreferences and Linkouts ( 0 )
References (1)