FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
pum, Not3
FlyBase ID
FBig0000121878
Interaction Network
Interactions Browser links
pum network
Not3 network
Reported Interactions
FBrf0254621-3.Y2H
Reference
Haugen et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pum 

Pumillo

bait

fused to GAL4 DNA-binding domain

pum 
pum 
Not3 

Not3

prey

fused to GAL4 activation domain

Not3 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pum 
RD3 (CR1 and CR4) domain
necessary binding region
aa 893-1037
pum 
RD3 domain residues 1033 and 1040
mutation decreasing interaction
aa 1033 and 1040

F1033A, F1040A

Not3 
C terminal domain
sufficient binding region
aa 522-849
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Haugen et al., 2022)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Haugen et al., 2022)
External Crossreferences and Linkouts ( 0 )
References (1)