FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Usp5, Atg1
FlyBase ID
FBig0000122925
Interaction Network
Interactions Browser links
Usp5 network
Atg1 network
Reported Interactions
FBrf0257321-1.coIP.WB
Reference
Pai et al., 2023
Description
physical association
Assay
Collection
Source/Stage
head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Usp5 

Leon

bait

FLAG tag

Atg1 

Atg1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult head of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Pai et al., 2023)
FBrf0257321-2.PD.WB
Reference
Pai et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Usp5 

Leon

bait

GST tag

Atg1 

Atg1

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293T cells; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected construct.

(Pai et al., 2023)
FBrf0257321-3.coIP.WB
Reference
Pai et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Usp5 

Leon

bait

HA tag

Usp5 
Usp5 
Atg1 

Atg1

prey

FLAG

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Usp5 
N-terminal domain
sufficient binding region
aa 1-297

coordinates relative to Usp5-PA

Usp5 
C-terminal domain
sufficient binding region
aa 297-827

coordinates relative to Usp5-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293T cells; bait and prey produced from transfected construct.

(Pai et al., 2023)
External Crossreferences and Linkouts ( 0 )
References (1)