FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Lpt, His3
FlyBase ID
FBig0000123100
Interaction Network
Interactions Browser links
Lpt network
His3 network
Reported Interactions
FBrf0257810-5.PD.MW
Reference
Zraly et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Lpt 

Cmi

bait

MBP tag

Lpt 
His3 

H3.2

prey

Biotin tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Lpt 
PHDb domain
sufficient binding region
aa 491-711
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; bait commercially synthesized.

(Zraly et al., 2023)
FBrf0257810-6.PD.MW
Reference
Zraly et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
His3 

H3.2

bait

Biotin tag

His3 
His3 
Lpt 

Cmi

prey

MBP tag

Lpt 
Lpt 
Lpt 
Lpt 
Lpt 
Lpt 
Lpt 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
His3 
tail peptides
sufficient binding region
aa 1-21
His3 
interaction domain
sufficient binding region
aa 22-44aa 44-63
Lpt 
PHDb.f3 domain
sufficient binding region
aa 653-711
Lpt 
cysteines
mutation decreasing interaction
aa 628,631,649,652

C628A, C631A, C649A, C652A

Lpt 
PHDb domain residue 542
mutation decreasing interaction
aa 542

D542K

Lpt 
PHDb domain residue 680
mutation decreasing interaction
aa 680

W680A

Lpt 
PHDb domain residue 669
mutation decreasing interaction
aa 669

E669A

Lpt 
PHDb domain residue 597
mutation decreasing interaction
aa 597

D597A

Lpt 
PHDb domain residue 600
mutation decreasing interaction
aa 600

D600A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Lpt-His3 interaction decreases with increasing number of Lpt mutated cysteines. Unspecified number of zinc finger spacer nucleotides required for His3 interaction with minimal Lpt PHDb.f3 domain.

(Zraly et al., 2023)

Interaction in vitro; bait commercially synthesized; prey produced as a recombinant fusion protein in bacterial system.

(Zraly et al., 2023)
FBrf0257810-7.ITC
Reference
Zraly et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Lpt 

Cmi

bait
His3 

H3.2

prey
His3 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
His3 
tail peptides
sufficient binding region
aa 1-21
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Zraly et al., 2023)
FBrf02578104-3.PD.WB
Reference
Zraly et al., 2023
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Lpt 

Cmi

bait

MBP tag

Lpt 
Lpt 
His3 

H3.2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Lpt 
PHDb.f3 domain
sufficient binding region
aa 653-711
Lpt 
cysteines
mutation decreasing interaction
aa 628,631,649,652

C628A, C631A, C649A, C652A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Zraly et al., 2023)

Lpt-His3 interaction decreases with increasing number of Lpt mutated cysteines.

(Zraly et al., 2023)
External Crossreferences and Linkouts ( 0 )
References (1)