FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Mmp1, NijA
FlyBase ID
FBig0000123187
Interaction Network
Interactions Browser links
Mmp1 network
NijA network
Reported Interactions
FBrf0192458-1.Y2H
Reference
Zhang et al., 2006
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stageorganism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mmp1 

Mmp1

bait

fused to GAL4 DNA-binding domain

Mmp1 
Mmp1 
NijA 

NijA

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mmp1 
hemopexin domain
sufficient binding region
aa 299-541

coordinates relative to Mmp1-PG, PF and PD.

Mmp1 
catalytically inactive
mutation increasing interaction
aa 225

E225A; coordinates relative to Mmp1-PG, PF and PD.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD. Text refers to Mmp1 isoform 1, which corresponds to PG, PF and PD isoforms. This full length isoform, whose catalytic domain has been mutated, or the hemopexin domain which lacks the catalytic domain, interacts with NijA.

(Zhang et al., 2006)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo and third instar larval cDNA expression library).

(Zhang et al., 2006)
FBrf0192458-2.coIP.WB
Reference
Zhang et al., 2006
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mmp1 

Mmp1

bait
Mmp1 
NijA 

NijA

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mmp1 
catalytically inactive residue 225
mutation increasing interaction
aa 225

E225A; coordinates relative to Mmp1-PG, PF and PD.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adults of transgenic fly line; bait produced from transgenic construct; prey produced from endogenous gene.

(Zhang et al., 2006)
FBrf0192458-3.coIP.WB
Reference
Zhang et al., 2006
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mmp1 

Mmp1

bait
NijA 

NijA

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adults of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Zhang et al., 2006)
External Crossreferences and Linkouts ( 0 )
References (1)