FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Tbp, Ubx
FlyBase ID
FBig0000123309
Interaction Network
Interactions Browser links
Tbp network
Ubx network
Reported Interactions
FBrf0260147-3.BiFC.FM
Reference
Villarreal-Puente et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tbp 

Tbp

bait

Venus tag, N-terminal fragment

Tbp 
Ubx 

Ubx

prey

Venus tag, C-terminal fragment

Ubx 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ubx 
N and C terminal domains
mutation decreasing interaction
aa 1-294 and 356-389
Tbp 
Poly Q domain
sufficient binding region
aa 245-363
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live HEK293 cells; proteins produced from transfected constructs.

(Villarreal-Puente et al., 2024)
FBrf0260147-5.FRET.FM
Reference
Villarreal-Puente et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tbp 

Tbp

fluorescence donor

CFP tag

Tbp 

Tbp

fluorescence donor

CFP tag

Tbp 

Tbp

fluorescence donor

CFP tag

Ubx 

Ubx

fluorescence acceptor

Venus tag

Ubx 

Ubx

fluorescence acceptor

Venus tag

Ubx 

Ubx

fluorescence acceptor

Venus tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Tbp forms a trimer with Antp-Ubx that experesses the Venus tag via bimolecular fluorescence complementation.

(Villarreal-Puente et al., 2024)

Source was live HEK293 cells; fluorescence donor produced from tagged transgenic construct; fluorescence acceptor produced from tagged transgenic construct.

(Villarreal-Puente et al., 2024)
External Crossreferences and Linkouts ( 0 )
References (1)