FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000123441
Interaction Network
Interactions Browser links
Su(var)3-9 network
His3 network
Reported Interactions
FBrf0144851-1.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

SU(VAR)3-9

enzyme

GST tag

SU(VAR)3-9

enzyme

GST tag

His3 

H3

enzyme target

[3]H methyl label

His3 

H3

enzyme target

[3]H methyl label

His3 
His3 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0180260-1.ENZ
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Su(var)3-9

enzyme

GST tag

Su(var)3-9

enzyme

GST tag

His3 

H3

enzyme target
His3 

H3

enzyme target
His3 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0204799-2.ENZ.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

SU(VAR)3-9

bait
His3 

His3

prey

[3]H label

His3 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
His3 
histone tail
necessary binding region
aa 1-20
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

Interaction in vitro; enzyme produced as a recombinant fusion protein in Sf9 baculovirus system; enzyme target chemically synthesized.

Su(var)3-9 methylates His3 tail at H3K9 in a manner that is inhibited by pre-existing H3K4 methylation.

FBrf0221240-7.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Su(var)3-9

enzyme

His tag

Su(var)3-9

enzyme

His tag

His3 

H3

enzyme target

[3]H label

His3 

H3

enzyme target

[3]H label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
External Crossreferences and Linkouts ( 0 )
References (4)