FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
CtsK1, psh
FlyBase ID
FBig0000123466
Interaction Network
Interactions Browser links
CtsK1 network
psh network
Reported Interactions
FBrf0261416-1.Y2H
Reference
Tang et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CtsK1 

CtsK1

bait

fused to GAL4 DNA-binding domain

CtsK1 
psh 

psh

prey

fused to GAL4 activation domain

psh 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
CtsK1 
C-terminal domain
sufficient binding region
aa 326-549

coordinates relative to CtsK1-PA

psh 
N-terminal cleavage site residues 144-147
necessary binding region
aa 144-147

IVGGY to AAAA; within the context of aa 23-148; coordinates relative to psh-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD. Addition of Fkp1 from Metarhizium robertsii disrupts CtsK1-psh interaction in a Y3H system.

(Tang et al., 2025)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Tang et al., 2025)
External Crossreferences and Linkouts ( 0 )
References (1)