FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
dom, Tbp
FlyBase ID
FBig0000123563
Interaction Network
Interactions Browser links
dom network
Tbp network
Reported Interactions
FBrf0261422-3.coIP.WB
Reference
Shen et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dom 

dEP400

bait

FLAG tag

dom 
dom 
Tbp 

TBP

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dom 
Middle region
sufficient binding region
aa 659-1934

coordinates relative to dom-PA

dom 
N-terminal region
sufficient binding region
aa 1-658

coordinates relative to dom-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Shen et al., 2025)

dom middle region interacts more strongly with Tbp than the N-terminal region.

(Shen et al., 2025)
FBrf0261422-4.coIP.WB
Reference
Shen et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tbp 

TBP

bait

FLAG tag

Tbp 
Tbp 
dom 

dEP400

prey

HA tag

dom 
dom 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Tbp 
C-terminal region
sufficient binding region
aa 176-354

coordinates relative to Tbp-PA

Tbp 
N-terminal phosphorylation residues
mutation decreasing interaction
aa 6, 16, 113, 161, 164, 167

S6A, T16A, T113A, T122A, T161A, T164A, T167A; coordinates relative to TBP-PA

dom 
Middle region
sufficient binding region
aa 659-1934

coordinates relative to dom-PA

dom 
N-terminal region
sufficient binding region
aa 1-658

coordinates relative to dom-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Shen et al., 2025)

dom middle and N-terminal regions interact more strongly with wild-type and phospormimetic Tbp indicating dom is preferentially recruited by phosphorylated Tbp.

(Shen et al., 2025)
External Crossreferences and Linkouts ( 0 )
References (1)