Various concentrations of exd were mixed with fluorescently labeled DNA. DNA-protein complexes observed by EMSA at higher concentrations resulted in a super shift of two exd protein molecules bound to DNA.

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

Dissociation constants were 146+/-13 nM for wild-type exd and 38+/-4 nM for G290A exd.