FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Trl, br
FlyBase ID
FBig0000123593
Interaction Network
Interactions Browser links
Trl network
br network
Reported Interactions
FBrf0260563-35.Y2H
Reference
Bonchuk et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
br 

Broad complex

bait

fused to GAL4 DNA-binding domain

br 
Trl 

GAF

prey

fused to GAL4 activation domain

Trl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Trl 
TTK-type BTB domain
sufficient binding region
aa 1-125
br 
TTK-type BTB domain
sufficient binding region
aa 1-121
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Bonchuk et al., 2024)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Bonchuk et al., 2024)
FBrf0260563-36.Y2H
Reference
Bonchuk et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Trl 

GAF

bait

fused to GAL4 DNA-binding domain

Trl 
br 

Broad complex

prey

fused to GAL4 activation domain

br 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Trl 
TTK-type BTB domain
sufficient binding region
aa 1-125
br 
TTK-type BTB domain
sufficient binding region
aa 1-121
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Bonchuk et al., 2024)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Bonchuk et al., 2024)
External Crossreferences and Linkouts ( 0 )
References (1)