FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
SkpA, Ppa
FlyBase ID
FBig0000123650
Interaction Network
Interactions Browser links
SkpA network
Ppa network
Reported Interactions
FBrf0131384-1.Y2H
Reference
Raj et al., 2000
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
SkpA 

Skp1

bait

fused to LexA DNA-binding domain

Ppa 

Ppa

prey

fused to LexA activation domain

Ppa 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ppa 
interaction domain
sufficient binding region
aa 131-538
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/LexA-AD

(Raj et al., 2000)

Fig4J

(Raj et al., 2000)

Source was yeast cell line; bait and prey produced as transgenic fusion proteins.

(Raj et al., 2000)
FBrf0131384-2.coIP.WB
Reference
Raj et al., 2000
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ppa 

Ppa

bait

HA tag

Ppa 
SkpA 

Skp1

prey

LexA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ppa 
interaction domain
sufficient binding region
aa 131-538
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell extracts; bait and prey produced from yeast transformed constructs.

(Raj et al., 2000)
External Crossreferences and Linkouts ( 0 )
References (1)