SoxN, SNCF Physical Interaction report

General Information

Interaction Type

physical association

Interacting Genes

SoxN, SNCF

FlyBase ID

FBig0000123665

Interaction Network

Interactions Browser links

SoxN network
SNCF network

Reported Interactions

FBrf0173145-1.Y2H

Reference

Bonneaud et al., 2003

Description

physical association

Assay

two hybrid

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

SoxN

bait

fused to GAL4 DNA-binding domain

SoxN

SNCF

prey

fused to GAL4 activation domain

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

SoxN

interaction domain

sufficient binding region

aa 240-572

contains 2/3 of the HMG domain and the complete C-terminal end; coordinates relative to SoxN-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Bonneaud et al., 2003)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Bonneaud et al., 2003)

FBrf0173145-2.PD.A

Reference

Bonneaud et al., 2003

Description

physical association

Assay

pull down

autoradiography

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

SNCF

bait

GST tag

SoxN

prey

[35]S label

SoxN

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

SoxN

HMG domain

sufficient binding region

aa 179-255

text states the HMG domain contains a few flanking, unspecified aa; coordinates relative to SoxN-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Bonneaud et al., 2003)

FBrf0173145-3.PD.A

Reference

Bonneaud et al., 2003

Description

physical association

Assay

pull down

autoradiography

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

SoxN

bait

GST tag

SNCF

prey

[35]S label

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Bonneaud et al., 2003)

FBrf0173145-4.Y2H

Reference

Bonneaud et al., 2003

Description

physical association

Assay

two hybrid

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

SNCF

bait

fused to GAL4 DNA-binding domain

SoxN

prey

fused to GAL4 activation domain

SoxN

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

SoxN

interaction domain

sufficient binding region

aa 240-572

contains 2/3 of the HMG domain and the complete C-terminal end; coordinates relative to SoxN-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Bonneaud et al., 2003)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Bonneaud et al., 2003)

External Crossreferences and Linkouts ( 0 )

References (1)

Report Sections

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