FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
lili, Mad
FlyBase ID
FBig0000123706
Interaction Network
Interactions Browser links
lili network
Mad network
Reported Interactions
FBrf0262581-1.Y2H
Reference
Roy et al., 2025
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
lili 

lili

bait

fused to GAL4 DNA-binding domain

lili 
Mad 

Mad

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
lili 
third intracellular loop
sufficient binding region
aa 219-352
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Roy et al., 2025)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Roy et al., 2025)
FBrf0262581-5.Y2H
Reference
Roy et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
lili 

lili

bait

fused to GAL4 DNA-binding domain

lili 
lili 
Mad 

Mad

prey

fused to GAL4 activation domain

Mad 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
lili 
C-terminal third intracellular loop
necessary binding region
aa 341-352

C-terminal deletion from fragment starting at aa 306.

lili 
N-terminal third intracellular loop
necessary binding region
aa 219-248

Nterminal deletion from fragment ending at aa 251.

Mad 
interaction domain
sufficient binding region
aa 72-436

coordinates relative to Mad-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Roy et al., 2025)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Roy et al., 2025)
External Crossreferences and Linkouts ( 0 )
References (1)