Interaction in vitro; bait and prey produced as recombinant fusion proteins in bacterial system.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from adult male testis cDNA expression library).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
