FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
mask, N
FlyBase ID
FBig0000123812
Interaction Network
Interactions Browser links
mask network
N network
Reported Interactions
FBrf0263071-1.Y2H
Reference
Sarkar et al., 2025
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
N 

Notch

bait

fused to LexA DNA-binding domain

N 
mask 

mask

prey

fused to GAL4 activation domain

mask 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
N 
Intracellular domain
sufficient binding region
aa 1896-2109

coordinates relative to N-PA

mask 
second ankyrin repeat
sufficient binding region
aa 2572-2718
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Sarkar et al., 2025)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Sarkar et al., 2025)
FBrf0263071-2.coIP.WB
Reference
Sarkar et al., 2025
Description
physical association
Assay
Collection
Source/Stage
wing disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mask 

mask

bait
N 

Notch

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was wing discs of transgenic fly line; bait and pret produced from transgenic constructs.

(Sarkar et al., 2025)
FBrf0263071-3.coIP.WB
Reference
Sarkar et al., 2025
Description
physical association
Assay
Collection
Source/Stage
wing disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
N 

Notch

bait
mask 

mask

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was wing discs of transgenic fly line; bait and prey produced from transgenic constructs.

(Sarkar et al., 2025)
External Crossreferences and Linkouts ( 0 )
References (1)