Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Prey was cloned reagent (previously isolated).
Two-hybrid system: yeast GAL4-BD/GAL4-AD.
Interaction in vitro; proteins produced in a baculovirus and insect cell system.
The CMG complex is captured as 55 pairwise direct or indirect interactions.
Interaction in vitro; bait produced as a recombinant fusion protein in a baculovirus and insect cell system; prey in a baculovirus and insect cell system.