Array of DNA fragments generated by PCR amplification; correspond to regions within postulated genes. Used for differential gene expression assays by hybridization to RNA. Technology type (GEO): spotted DNA/cDNA.
DGRC-1 amplicon transcriptome microarrays are spotted with DNA fragments amplified from genomic DNA using gene-specific oligonucleotide primer pairs. The primers were made by Incyte Genomics and were generously donated to the DGRC by Incyte Genomics and Brian Oliver (NIDDK, National Institutes of Health). The resulting microarray consists of 15,552 spots corresponding to 88% of the genes in the Drosophila melanogaster annotation release 4.3.
Genomic location data received from James Costello on May 11 2006 as a curated list of coordinates based on BLAST analysis of predicted amplicons against Dmel release 4; migrated to release 5 by FlyBase.