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General Information
Name
DGRC-1
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000027
Project
    Created by
    Vector
      Title
      A gene expression microarray consisting of spotted PCR-amplified genomic DNA corresponding to regions within annotated genes, DGRC.
      Overview
      Description

      Array of DNA fragments generated by PCR amplification; correspond to regions within postulated genes. Used for differential gene expression assays by hybridization to RNA. Technology type (GEO): spotted DNA/cDNA.

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      No longer available
      Biosample Source
      Overview
      Strain
      Stage
      Sex
      Tissue isolated
      Other tissues studied
      Cell component
      Cell line
      Key genes
      Methods
      Sample preparation
      Reagent Details
      Methods
      Protocol

      DGRC-1 amplicon transcriptome microarrays are spotted with DNA fragments amplified from genomic DNA using gene-specific oligonucleotide primer pairs. The primers were made by Incyte Genomics and were generously donated to the DGRC by Incyte Genomics and Brian Oliver (NIDDK, National Institutes of Health). The resulting microarray consists of 15,552 spots corresponding to 88% of the genes in the Drosophila melanogaster annotation release 4.3.

      Mode of Assay
      Data analysis
      Comments

      Genomic location data received from James Costello on May 11 2006 as a curated list of coordinates based on BLAST analysis of predicted amplicons against Dmel release 4; migrated to release 5 by FlyBase.

      Associated Data
      Size
      Files
      Additional Information
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      DGRC-1
      DGRC-D. melanogaster-DGRC1-15552
      GEO Platform GPL3830
      Name Synonyms
      A gene expression microarray consisting of spotted PCR-amplified genomic DNA corresponding to regions within annotated genes, DGRC.
      Secondary FlyBase IDs
        References (1)