FB2026_03 , released September 17, 2026
Result: FlyAt_L3.M_tubule
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General Information
Name
FlyAt_L3.M_tubule
Species
D. melanogaster
Result type
gene expression profile
FlyBase ID
FBlc0000179
Project
Data Provider
Title
Gene expression values from microarray analysis of D. melanogaster, Canton-S strain, larval Malpighian tubule.
Status
Current
Accessions
    Biosample Source
    Overview
    Strain
    Canton-S
    (Chintapalli et al., 2010.9.17)
    Stage
    larval stage
    (Chintapalli et al., 2010.9.17)
    Sex
    Tissue isolated
    embryonic/larval Malpighian tubule
    (Chintapalli et al., 2010.9.17)
    Other tissues studied
    Cell component
    Cell line
    Key genes
    Methods
    Sample preparation

    Tissue source: dissected Malpighian tubules (anterior and posterior tubules with their common ureters, severed at the junction of the gut) of early third instar larvae.

    Canton-S feeding third instar larvae were reared at 22oC on a 12:12hr light:dark regime on standard Drosophila diet. Tissues were isolated by hand dissection from equal numbers of male and female flies (except in the case of gonads). Multiple samples were pooled to obtain enough total RNA.

    Biosamples analyzed by this result (1)
    Biosample
    Type
    Title
    BS_FlyAt_L3_M_tubule
    tissue
    D. melanogaster, Canton-S strain, larval Malpighian tubule, source for RNA.
    Data Analyzed
    Assay
    RNA expression microarray
    (Chintapalli et al., 2010.9.17)
    Assay methods
    Affymetrix Gene Expression Array 2
    (Chintapalli et al., 2010.9.17)
    total RNA isolation
    (Chintapalli et al., 2010.9.17)
    Key genes
    Protocol

    Total RNA was extracted by RNEasy Micro kit (Invitrogen). 2ug of total RNA was used for labeled cRNA synthesis, according to Affymetrix protocol.

    Mode of Assay

    Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45[o]C on GeneChip Drosophila Genome 2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450. GeneChips were scanned using the GeneChip scanner 3000 7G.

    Raw Data Analyzed (1)
    Assay / Reagent collection
    Type
    Title
    Microarray_FlyAt_L3_malpighian_tubule
    RNA expression microarray
    Expression microarray analysis of D. melanogaster, Canton-S strain, larval Malpighian tubule.
    Processed Data Analyzed (0)
    Result
    Type
    Title
    Analysis
    Methods
    Reference Genome
    Reference Annotation
    Data analysis

    The data were analyzed with GCOS1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100. For each tissue, the entire procedure was repeated 4 times, to create 4 independent replicates.

    Comments
    Associated Data
    Size
    Files
    Additional Information
    Synonyms and Secondary IDs (4)
    Reported As
    Symbol Synonym
    FlyAt_L3.M_tubule
    Larval Tubule
    (Chintapalli et al., 2010.9.17)
    Larval tubule
    (Chintapalli et al., 2010.9.17)
    Name Synonyms
    Gene expression values from microarray analysis of D. melanogaster, Canton-S strain, larval Malpighian tubule.
    (Chintapalli et al., 2010.9.17)
    Secondary FlyBase IDs
      References (1)