FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Reagent: TRiP-5
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General Information
Name
TRiP-5
Species
D. melanogaster
Reagent type
RNAi construct collection
FlyBase ID
FBlc0000416
Project
Created by
DRSC
Vector
Title
A set of transgenic RNAi constructs for expression of shRNA under UAS control, TRiP second generation (pVALIUM21).
Accessions
    Overview
    Description

    The TRiP-5 construct collection represents a second generation of TRiP lines. Each construct is designed to target the knockdown of a single gene during oogenesis using short hairpin RNA (shRNA).

    (Ni et al., 2011.7.19)

    NOTE: Dataset members correspond to the amplicon sequence features; these can be used to retrieve associated constructs and stocks.

    (FlyBase Curators, 2013)
    Available from
    Bloomington Drosophila Stock Center
    Biosample Source
    Overview
    Strain
    Stage
    Sex
    Tissue isolated
    Other tissues studied
    Cell component
    Cell line
    Key genes
    Methods
    Sample preparation
    Reagent Details
    Methods
    Molecular Construct used
    Transgenic Construct used
    Protocol

    Transgenic animals were designed to carry a short hairpin (shRNA) microRNA under UAS-GAL4 control. These shRNA are inserted into pVALIUM21, which is a variant of pVALIUM22 (TRiP-4) that lacks the ftz intron and gypsy sequences.

    (Ni et al., 2011.7.19)
    Mode of Assay
    Data analysis
    Comments

    Constructs of the TRiP-5 collection, based on pVALIUM21, are designed to trigger effective RNAi knock-down in the female germline. The P-transposase core promoter in pVALIUM21-based constructs directs little expression in somatic cells.

    (Ni et al., 2011.7.19)

    Many of the TRiP RNAi and sgRNA stocks generated at Harvard Medical School (HMS lines) and Tsinghua University (HMC lines), but not stocks generated at the National Institute of Genetics in Japan (HMJ lines), contain the sev21 allele as a result of introducing a mutated X chromosome from Bloomington stock 35781 (FBst0035781) or 32261 (FBst0032261) as the transgenes were being established in stock.

    (Escobedo et al., 2019)
    Associated Data
    Size
    Associated features
    94 RNAi_reagent(s)
    Download 
    Files
    Additional Information

    [TRiP Approach - 2nd generation](http://www.flyrnai.org/TRiP-TTR-GEN2.html)

    Synonyms and Secondary IDs (3)
    Reported As
    Symbol Synonym
    TRiP-5
    (Schroeder, 2014.6.12)
    VALIUM21
    (Zirin et al., 2020)
    Name Synonyms
    A set of transgenic RNAi constructs for expression of shRNA under UAS control, TRiP second generation (pVALIUM21).
    (FlyBase Curators, 2016-)
    Secondary FlyBase IDs
      References (6)