The TRiP-5 construct collection represents a second generation of TRiP lines. Each construct is designed to target the knockdown of a single gene during oogenesis using short hairpin RNA (shRNA).
NOTE: Dataset members correspond to the amplicon sequence features; these can be used to retrieve associated constructs and stocks.
Constructs of the TRiP-5 collection, based on pVALIUM21, are designed to trigger effective RNAi knock-down in the female germline. The P-transposase core promoter in pVALIUM21-based constructs directs little expression in somatic cells.
Many of the TRiP RNAi and sgRNA stocks generated at Harvard Medical School (HMS lines) and Tsinghua University (HMC lines), but not stocks generated at the National Institute of Genetics in Japan (HMJ lines), contain the sev21 allele as a result of introducing a mutated X chromosome from Bloomington stock 35781 (FBst0035781) or 32261 (FBst0032261) as the transgenes were being established in stock.
[TRiP Approach - 2nd generation](http://www.flyrnai.org/TRiP-TTR-GEN2.html)