5'-ends of capped transcripts were isolated by 5'-SAGE. Briefly, a biotinylated adapter to 5'-phosphate ends exposed after cleavage of the 5'-cap by tobacco acid pyrophosphatase (TAP). Double-stranded cDNAs were then cleaved with MmeI to generate short 25-27nt fragments, and biotinylated adapters (along with adjacent 5'end sequence) are purified. These SAGE fragments were then used for Illumina library construction (single end layout).
Read length (bases): 25-27
Total reads: 51,764,976
Total reads: 6,711,841
Uniquely aligned reads: 25,083,481
Uniquely aligned reads: 3,556,021
Reads were mapped to Dmel genome annotation R5.3 using a parallel version of BLAT. Only uniquely mapped reads were considered (allowing for up to 3 mismatches). Associated TSS profiles represent mapped 5'-ends.
