A BAC library containing sheared genomic DNA fragments with average size of 48 kb.
DNA was isolated from adult fly tissue, embedded in agarose blocks and sheared by four freeze/thaw cycles. Ends were polished by treatment with mung bean nuclease and T4 DNA polymerase, and cloned into the pTARBAC6 vector between the BstXI sites.
Though 17,992 clones were generated, sequences for only a few have been deposited at NCBI.