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General Information
Name
CHORI-321_BAC
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000783
Project
Created by
Vector
    Title
    Pacman genomic clone collection for D. melanogaster, iso-1 strain, adult, 83kb inserts.
    Accessions
    Overview
    Description

    A BAC library containing genomic DNA fragments (average size of 83 kb) in the attB-P[acman]-CmR-BW vector for efficient transgenesis.

    Available from
    [Pacman Libraries](http://flypush.imgen.bcm.tmc.edu/pacmanfly/)
    Biosample Source
    Overview
    Sex
    Tissue isolated
    Other tissues studied
    Cell component
    Cell line
    Key genes
    Methods
    Sample preparation
    Biosamples used (1)
    Biosample
    Type
    Title
    D. melanogaster, iso-1 strain, adult, source for BAC genomic clones.
    Reagent Details
    Protocol

    DNA isolated from adult fly tissue was prepared in agarose blocks and partially digested with MboI. 80-100 kb fragments were size-selected by contour-clamped homogeneous electric field (CHEF) gel electrophoresis, and ligated into the BamHi site of the attB-P[acman]-CmR-BW vector, a special adaptation of the P[acman] vector. Libraries were transformed into the E.coli strain "EPI300-T1R".

    Mode of Assay

    The ends of the BAC inserts were sequenced (paired ends) and aligned to the reference genome assembly.

    Data analysis
    Comments

    A total of 36,864 clones were generated.

    Associated Data
    Size
    Associated features
    12,456 Clone(s)
    Files
    Additional Information
    Synonyms and Secondary IDs (3)
    Reported As
    Symbol Synonym
    CHORI-321_BAC
    Name Synonyms
    Pacman genomic clone collection for D. melanogaster, iso-1 strain, adult, 83kb inserts.
    Secondary FlyBase IDs
      References (3)