FB2026_03 , released September 17, 2026
Assay: CAGE-Seq_MachiBase_AF_young
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General Information
Name
CAGE-Seq_MachiBase_AF_young
Species
D. melanogaster
Assay type
CAGE-Seq
FlyBase ID
FBlc0000942
Project
Data Provider
Title
CAGE-Seq of capped transcript 5' ends from D. melanogaster, Canton-S strain, young adult female.
Accessions
Biosample Source
Overview
Strain
Canton-S
(Ahsan et al., 2009)
Stage
adult stage
(Ahsan et al., 2009)
Sex
female
(Ahsan et al., 2009)
Tissue isolated
organism
(Ahsan et al., 2009)
Other tissues studied
Cell component
Cell line
Key genes
Methods
Sample preparation
Biosamples used (1)
Biosample
Type
Title
BS_MachiBase_AdF_young
whole organism
D. melanogaster, Canton-S strain, young adult female, source for RNA.
Assay Details
Methods
single-end layout
(Ahsan et al., 2009)
Illumina sequencing
(Ahsan et al., 2009)
Key genes
Protocol

5'-ends of capped transcripts were isolated by 5'-SAGE. Briefly, a biotinylated adapter to 5'-phosphate ends exposed after cleavage of the 5'-cap by tobacco acid pyrophosphatase (TAP). Double-stranded cDNAs were then cleaved with MmeI to generate short 25-27nt fragments, and biotinylated adapters (along with adjacent 5'end sequence) are purified. These SAGE fragments were then used for Illumina library construction (single end layout).

Mode of Assay

Read length (bases): 25-27

(Ahsan et al., 2009)

Total reads: 51,764,976

Uniquely aligned reads: 25,083,481

Comments
Associated Data
Size
6,882,149 reads
(Ahsan et al., 2009)
Files
Additional Information
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
CAGE-Seq_MachiBase_AF_young
Name Synonyms
CAGE-Seq of capped transcript 5' ends from D. melanogaster, Canton-S strain, young adult female.
(Ahsan et al., 2009)
Secondary FlyBase IDs
    References (2)