FB2026_03 , released September 17, 2026
Assay: Microarray_FlyAt_L3_CNS
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General Information
Name
Microarray_FlyAt_L3_CNS
Species
D. melanogaster
Assay type
RNA expression microarray
FlyBase ID
FBlc0000990
Project
Data Provider
Title
Expression microarray analysis of D. melanogaster, Canton-S strain, larval central nervous system.
Accessions
Biosample Source
Overview
Strain
Canton-S
(Chintapalli et al., 2010.9.17)
Stage
larval stage
(Chintapalli et al., 2010.9.17)
Sex
Tissue isolated
larval central nervous system
(Chintapalli et al., 2010.9.17)
Other tissues studied
Cell component
Cell line
Key genes
Methods
Sample preparation

Tissue source: dissected central nervous systems (brain and thoracic ganglia) of early third instar larvae.

Canton-S feeding third instar larvae were reared at 22oC on a 12:12hr light:dark regime on standard Drosophila diet. Tissues were isolated by hand dissection from equal numbers of male and female flies (except in the case of gonads). Multiple samples were pooled to obtain enough total RNA.

Biosamples used (1)
Biosample
Type
Title
BS_FlyAt_L3_CNS
tissue
D. melanogaster, Canton-S strain, larval central nervous system, source for RNA.
Assay Details
Methods
total RNA isolation
(Chintapalli et al., 2010.9.17)
Affymetrix Gene Expression Array 2
(Chintapalli et al., 2010.9.17)
Key genes
Protocol

Total RNA was extracted by RNEasy Micro kit (Invitrogen). 2ug of total RNA was used for labeled cRNA synthesis, according to Affymetrix protocol.

Mode of Assay

Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45[o]C on GeneChip Drosophila Genome 2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450. GeneChips were scanned using the GeneChip scanner 3000 7G.

Comments
Associated Data
Size
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Additional Information
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Microarray_FlyAt_L3_CNS
Name Synonyms
Expression microarray analysis of D. melanogaster, Canton-S strain, larval central nervous system.
(Chintapalli et al., 2010.9.17)
Secondary FlyBase IDs
    References (2)