FB2026_03 , released September 17, 2026
Assay: ChIP-Seq_Furlong_H3K27me3_E11-12_mesoderm
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General Information
Name
ChIP-Seq_Furlong_H3K27me3_E11-12_mesoderm
Species
D. melanogaster
Assay type
ChIP-Seq
FlyBase ID
FBlc0001002
Project
Data Provider
Title
Chip-Seq of H3K27me3 from D. melanogaster, embryonic mesodermal nuclei (6-8 hr AEL).
Accessions
Biosample Source
Overview
Strain
Stage
early extended germ band stage
(Bonn et al., 2012)
late extended germ band stage
(Bonn et al., 2012)
Sex
Tissue isolated
mesoderm
(Bonn et al., 2012)
Other tissues studied
Cell component
nucleus
(Bonn et al., 2012)
Cell line
Key genes
Methods
targeted cell labeling
(Bonn et al., 2012)
Sample preparation

A strain expressing mesodermally expressed SBP-tagged His2B was used to label nuclei for subsequent fluorescent "cell" sorting. Two independent biological replicates were used.

Biosamples used (1)
Biosample
Type
Title
BS_Furlong_E6-8h_mesoderm
isolated cells
D. melanogaster, embryonic mesodermal nuclei (6-8 hr AEL), source for chromatin.
Assay Details
Methods
single-end layout
(Bonn et al., 2012)
Illumina sequencing
(Bonn et al., 2012)
Key genes
(bait_protein)
Protocol

Chromatin immunoprecipitation of His3 H3K27me3 modification.

(Bonn et al., 2012)

Chromatin was prepared from formaldehyde-fixed embryos as previously described (FBrf0202481).

Mode of Assay

Chromatin immunoprecipitated DNA was analyzed by next generation sequencing.

Comments
Associated Data
Size
Files
Additional Information
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
ChIP-Seq_Furlong_H3K27me3_E11-12_mesoderm
Name Synonyms
Chip-Seq of H3K27me3 from D. melanogaster, embryonic mesodermal nuclei (6-8 hr AEL).
(Bonn et al., 2012)
Secondary FlyBase IDs
    References (2)