Canton-S flies were reared at 22oC on a 12:12hr light:dark regime on standard Drosophila diet, 1 week after adult emergence. Tissues were isolated by hand dissection from equal numbers of male and female flies (except in the case of gonads). Multiple samples were pooled to obtain enough total RNA.

Tissue source: dissected adult heart (the dorsal abdominal heart, with some adherent tissues, such as fat body).