Canton-S feeding third instar larvae were reared at 22oC on a 12:12hr light:dark regime on standard Drosophila diet. Tissues were isolated by hand dissection from equal numbers of male and female flies (except in the case of gonads). Multiple samples were pooled to obtain enough total RNA.

Tissue source: dissected trachae (the major tracheal trunks, excluding spiracles and obvious adherent tissues) of early third instar larvae.