A characterization of the diverse populations of cells in the adult Drosophila brain, upon alcohol exposure at the embryonic stage.
Reads were processed using Cell Ranger v3.1 (10x Genomics). They were aligned against the Drosophila melanogaster reference genome Release 6 plus ISO1 MT (GCA_000001215.4). Further analysis was perfored using the Seurat v3 R package. Counts were normalized by regularized negative binomial regression (scTransform). RunUMAP and FindNeighbors functions were used with 10 dimensions to ordinate expression space and reduce data dimensionality. Unsupervised clustering was performed using the FindClusters function.
The EMBL-EBI's Single Cell Expression Atlas provides cell-level annotations, clustering data, raw and normalised read counts, and putative marker genes.
