A characterization of the diverse populations of cells in the adult Drosophila brain, upon cocaine exposure.
Reads were processed using Cell Ranger v3.1 (10x Genomics). They were aligned against the Drosophila melanogaster reference genome Release 6 (GCA_000001215.4). Further analysis was performed using the Seurat v3 R package. Counts were filtered to exclude genes expressed in less than 5 cells and cells with less than 300 or more than 2,500 RNA features. Cell type clusters were identified by unsupervised clustering with a resolution of 0.8.
The EMBL-EBI's Single Cell Expression Atlas provides cell-level annotations, clustering data, raw and normalised read counts, and putative marker genes.
