FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Result: scRNAseq_2021_Baker_COCA_MIXEDSEX_seq_clustering
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General Information
Name
scRNAseq_2021_Baker_COCA_MIXEDSEX_seq_clustering
Species
D. melanogaster
Result type
cell clustering analysis
FlyBase ID
FBlc0005590
Project
Data Provider
Title
Clustering analysis of dissociated cells from adult brains upon cocaine exposure
Status
Current
Accessions
    Biosample Source
    Overview
    Strain
    Canton-S
    (Baker et al., 2021)
    Stage
    adult stage
    (Baker et al., 2021)
    Sex
    male
    (Baker et al., 2021)
    female
    (Baker et al., 2021)
    Tissue isolated
    adult brain
    (Baker et al., 2021)
    Other tissues studied
    Cell component
    Cell line
    Key genes
    Methods
    chemical treatment
    (Baker et al., 2021)
    multi-individual sample
    (Baker et al., 2021)
    tissue dissection
    (Baker et al., 2021)
    Sample preparation

    One hundred flies were allowed to age for 3 to 5 days on standard cornmeal/yeast/molasses-agar culture medium before being fed a 4% sucrose solution supplemented with 1ug/uL cocaine for at most 2 hours. Brains from the first 40 flies that consumed 0.53uL of the sucrose/cocaine solution were then dissociated with collagenase and stepwise trituration.

    Biosamples analyzed by this result (2)
    Biosample
    Type
    Title
    scRNAseq_2021_Baker_COCA_FEMALE
    isolated cells
    Dissociated cells from female adult brains upon cocaine exposure
    scRNAseq_2021_Baker_COCA_MALE
    isolated cells
    Dissociated cells from male adult brains upon cocaine exposure
    Data Analyzed
    Assay
    single-cell RNA-Seq
    (Baker et al., 2021)
    Assay methods
    10x sequencing
    (Baker et al., 2021)
    Key genes
    Protocol
    Mode of Assay
    Raw Data Analyzed (2)
    Assay / Reagent collection
    Type
    Title
    scRNAseq_2021_Baker_COCA_FEMALE_seq
    single-cell RNA-Seq
    Single-cell RNA-seq of dissociated cells from female adult brains upon cocaine exposure
    scRNAseq_2021_Baker_COCA_MALE_seq
    single-cell RNA-Seq
    Single-cell RNA-seq of dissociated cells from male adult brains upon cocaine exposure
    Processed Data Analyzed (0)
    Result
    Type
    Title
    Analysis
    Methods
    EMBL-EBI SCEA standard analysis method
    (Baker et al., 2021)
    Reference Genome
    Reference Annotation

    Dmel R6.32

    (Baker et al., 2021)
    Data analysis
    Comments
    Associated Data
    Size
    44764 cells
    (Baker et al., 2021)
    Files
    Additional Information
    Synonyms and Secondary IDs (2)
    Reported As
    Symbol Synonym
    scRNAseq_2021_Baker_COCA_MIXEDSEX_seq_clustering
    Name Synonyms
    Clustering analysis of dissociated cells from adult brains upon cocaine exposure
    (Baker et al., 2021)
    Secondary FlyBase IDs
      References (1)