FB2026_03 , released September 17, 2026
Recombinant Construct: pCFD5-white
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General Information
Symbol
pCFD5-white
FlyBase ID
FBms0003966
Feature type
cloned_region
Component Allele(s)
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Description

The pCFD5-white vector is designed to facilitate cloning of one or multiple guide RNAs (gRNAs) downstream of the strong, ubiquitous U6:3 (snRNA:U6:96Ac) promoter. The gRNAs are flanked by tRNAs and are initially expressed as a single polycistronic tRNA-gRNA cassette; after this primary transcript is expressed, the tRNAs should form secondary structures that are recognized by the endogenous tRNA-processing machinery (RNase enzymes P and Z), resulting in the release of the gRNAs (PMID:25733849). The vector contains an attB site, allowing integration into an attP-containing docking site in the genome to produce a transgenic construct.

(Port et al., 2014-)
Construct components
Component
Use(s)
Also carries
attB
Marker(s)
w+*
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
pCFD5-white
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (2)