Henikoff, S., Tatchell, K., Hall, B.D., Nasmyth, K.A. (1981). Isolation of a gene from Drosophila by complementation in yeast. Nature 289(5793): 33--37.
FlyBase ID
FBrf0037147
Publication Type
Research paper
Abstract
Transformation of mutant yeast cells by cloned genomic DNA from a higher eukaryote has made it possible to isolate a Drosophila DNA sequence that complements a yeast adenine-8-mutation. A 0.8-kilobase poly(A)-containing RNA is transcribed from the cloned Drosophila segment in transformed yeast cells and can account for functional expression of the gene.
