Gregory, R.J., Kammermeyer, K.L., Vincent, W.S., Wadsworth, S.G. (1987). Primary sequence and developmental expression of a novel Drosophila melanogaster Src gene.  Mol. Cell. Biol. 7(): 2119--2127.

FBrf0046962

Research paper

We have sequenced a cDNA clone for the Drosophila melanogaster gene Dsrc28C, a homolog of the vertebrate gene c-src. The cDNA contains a single open reading frame encoding a protein of 66 kilodaltons which contains features highly conserved within the src family of tyrosine protein kinases. Novel structural features of the Dsrc28C protein include a basic pI and a polyglycine domain near the amino terminus. Cell-free translation of in vitro-transcribed RNA yielded a protein of the predicted size which could be immunoprecipitated by anti-v-src antisera. RNA blot hybridization revealed that the gene is expressed predominantly during embryogenesis, in imaginal disks of third-instar larvae, and in adult females. In situ hybridization showed that expression in adult females is largely confined to nurse cells and developing oocytes.

PMC365333 (PMC) (EuropePMC)