Varadi, A., Gilmore-Hebert, M., Benz, E.J. (1989). Amplification of the phosphorylation site-ATP-binding site cDNA fragment of the Na⁺, K(+)-ATPase and the Ca²⁺-ATPase of Drosophila melanogaster by polymerase chain reaction.  FEBS Lett. 258(): 203--207.

FBrf0049751

Research paper

In vitro DNA-amplification technique has been utilized to generate a 430 bp fragment of the Na+,K(+)-ATPase, and a 550 bp fragment of a Ca2(+)-ATPase (the sarcoplasmic reticulum-type) of Drosophila melanogaster. The oligonucleotide primers for the DNA-amplification (Polymerase Chain Reaction) had been designed on the basis of amino acid sequence motifs--the phosphorylation site and the ATP-binding site--conserved among members of the ATPase protein family. Using the amplified cDNA-segments as probes, we demonstrated that there is one Na+,K(+)-ATPase and one Ca2(+)-ATPase (sarcoplasmic reticulum-type) gene in the Drosophila genome. Three different mRNA species are processed from the Na+,K(+)-ATPase gene and one from the Ca2(+)-ATPase gene. Developmental control in expression of the Ca2(+)-ATPase gene was observed.