Maki, C.G., Rhoads, D.D., Diaz, J.J., Roufa, D.J. (1990). A Drosophila ribosomal protein functions in mammalian cells. Mol. Cell. Biol. 10(): 4524--4528.
FlyBase ID
FBrf0052563
Publication Type
Research paper
Abstract
A cDNA expression vector encoding Drosophila ribosomal protein S14 was transfected into cultured Chinese hamster ovary (CHO) cells that harbor a recessive RPS14 emetine resistance mutation. Transformants synthesized the insect mRNA and polypeptide and consequently displayed an emetine-sensitive phenotype. These observations indicate that the insect protein was accurately expressed and correctly assembled into functional mammalian 40S ribosomal subunits.
