FB2025_01 , released February 20, 2025
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Citation
Ozyhar, A., Strangmann-Diekmann, M., Kiltz, H.H., Pongs, O. (1991). Characterization of a specific ecdysteroid receptor-DNA complex reveals common properties for invertebrate and vertebrate hormone-receptor/DNA interactions.  Europ. J. Biochem. 200(): 329--335.
FlyBase ID
FBrf0053926
Publication Type
Research paper
Abstract
A gel-filtration assay has been developed with which the specific interaction between Drosophila melanogaster ecdysteroid receptor and the 20-hydroxy-ecdysone responsive element of the hsp27 gene promoter region was characterized in terms of complex formation, saturation of DNA binding and the apparent molecular mass of the complex. The hsp27 DNA-binding sequence for ecdysteroid receptor in vitro was delimited by footprinting and mutational analysis. The combined results show that ecdysteroid receptor binds as a dimer to an imperfect palindromic sequence (GGTTCAATGCACT) closely resembling the structures of the different vertebrate steroid-hormone-responsive elements reported so far.
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Europ. J. Biochem.
    Title
    European Journal of Biochemistry
    Publication Year
    1967-2004
    ISBN/ISSN
    0014-2956
    Data From Reference
    Genes (2)
    Sequence Features (1)