Tseng, J.C., Zollman, S., Chain, A.C., Laski, F.A. (1991). Splicing of the Drosophila P-element ORF2-ORF3 intron is inhibited in a human cell extract.  Mech. Dev. 35(): 65--72.

FBrf0054625

Research paper

P element transposition in Drosophila melanogaster is regulated by germline-specific splicing of the P element ORF2-ORF3 intron. This regulation has been shown to depend on a cis-acting sequence located in the exon 12-31 bases from the 5' splice site. Mutations within this sequence disrupt the regulation and result in splicing of the ORF2-ORF3 intron in all tissues, indicating that the sequence is required to inhibit splicing of this intron in the soma. We now show that a trans-acting factor in a human (HeLa) cell extract can inhibit splicing of the intron, suggesting that this regulatory mechanism is conserved from flies to humans.