Lockett, T.J., Lewy, D., Holmes, P., Medveczky, K.M., Saint, R. (1992). The rough (ro++) gene as a dominant P-element marker in germ line transformation of Drosophila melanogaster. Gene 114(): 187--193.
FlyBase ID
FBrf0056088
Publication Type
Research paper
Abstract
We describe a new vector for the P-element-mediated introduction of gene constructs into the germ line of Drosophila melanogaster. The P-element vector carries 6.8 kb of genomic DNA containing the rough gene (ro) from D. melanogaster and a polylinker (MCS) containing ten unique cloning sites. To demonstrate its utility, we have cloned into the MCS of this vector, the firefly luciferase (Luc)-encoding gene (luc) under the control of the D. melanogaster hsp70 promoter and have transformed flies with the resultant P-element. Single insertions of this element, whether in the hemizygous or homozygous condition, completely rescued the ro- mutation and directed heat-inducible synthesis of Luc mRNA and enzyme.
