Subject: Re: help FlyBase & feel good Hi Mike, My appologies, here is a list of the chromosomes that I checked personally (I note the ones I still keep): OTHER CHROMOSOMES I CHECKED Df(3R)P14 90B3-8 to 91B1-2 (Ed Lewis chromosome-he has it end in 91A) Df(3R)cha-m7 90E-F to 91F5-13 MY GLASS MUTANT CHROMOSOMES Note: the mutagenized fly was a trans heterozygote between P(w+) A2-1 (a lethal insert at 91C) and P(w+)D3 (a viable, at 90E-F). Thus many of the chromosomes bear A2-1, and therefore that additional lethal locus. Df(3R)gl-BX1 gl- and lethal with itself, and P(w+)A2-1, but no cytologically or Southern blot visible aberation Df(3R)gl-BX2 gl- and lethal with itself, and P(w+)A2-1, but no cytologically or Southern blot visible aberation (I KEEP THIS STOCK) Df(3R)gl-BX3 90F to 91A3-8 (I KEEP THIS STOCK) Df(3R)gl-BX4 90F to 91A3-8 (probably the same as BX3, came from the same cross) (I KEEP THIS STOCK) Df(3R)gl-BX5 small, viable deletion (~4kb), within glass (I KEEP THIS STOCK) In(3R)gl-BX6 breaks are 84E and 91A, homozygous lethal, but viable over BX3, thus lethality is likely to be associated with the 84E breakpoint. (I KEEP THIS STOCK) Df(3R)gl-BX7 gl- and lethal with itself, and P(w+)A2-1, but no cytologically or Southern blot visible aberation (I KEEP THIS STOCK) Df(3R)gl-BX8 9011 to 91A6-8 also lethal with A2-1 (I KEEP THIS STOCK) Df(3R)gl-BX9 gl- and lethal with itself, viable with P(w+)A2-1, but no cytologically or Southern blot visible aberation (I KEEP THIS STOCK) Df(3R)gl-BX10 90C7-8 to 91B1 gl- and lethal with itself, and P(w+)A2-1 Df(3R)gl-BX11 gl- and lethal with itself, viable with P(w+)A2-1, but no cytologically or Southern blot visible aberation Df(3R)gl-BX12 90D3-6 to 91B5-8 gl- and lethal with itself, and P(w+)A2-1 Df(3R)gl-BX13 gl- and lethal with itself, viable with P(w+)A2-1, but no cytologically or Southern blot visible aberation Df(3R)gl-BX14 gl- and lethal with itself, viable with P(w+)A2-1, but no cytologically or Southern blot visible aberation OTHER (GLASS+) CHROMOSOMES FROM THE SAME SCREEN (DETECTED AS LOSS OF W+) l(3)BX1 gl+, lethal with itself, and P(w+)A2-1, but no cytologically or Southern blot visible aberation l(3)BX2 gl+, lethal with itself, and P(w+)A2-1, but no cytologically or Southern blot visible aberation l(3)BX3 gl+, lethal with itself, and P(w+)A2-1, but no cytologically or Southern blot visible aberation l(3)BX4 gl+, lethal with itself, and P(w+)A2-1, but no cytologically or Southern blot visible aberation l(3)BX5 91B1-2 to 91D1-2 gl+, lethal with itself, and P(w+)A2-1 l(3)BX6 90C9-10 to 90F2-11 gl+, lethal with itself,viable with P(w+)A2-1 In(3R)BX7 91B1-2 to 93F11-14 gl+, lethal with itself, and P(w+)A2-1 I left all these stocks with Gerry, I don't know which he still keeps. Also Don Gailey had his similar collection from this region. All the best, Kevin Kevin Moses at USC