dear Eleanor sorry. The Cahsneo vector you refer to is only fleetingly described in the article: Pirrotta, V. (1988) Vectors for P-mediated transformation in Drosophila. In: Vectors. A survey of molecular cloning vectors and their uses. Eds. R.L. Rodriguez and D.T. Denhardt. Butterworths. Boston. pp. 437-456. The brief description of Cahsneo and some vectors based on it are on p. 445 and p. 448. The Cahsneo with hs43-lacZ is described (also sketchily) in: Qian, S., Varjavand, B. and Pirrotta, V. (1992) Molecular analysis of the zeste-white interaction reveals a promoter-proximal element essential for distant enhancer-promoter interaction. Genetics 131:79-90 It contains not the full hsp70 promoter but just the TATA box, with sequences upstream of -43 deleted. The article in the Vectors book describes the cosPneo vector but concerning the cosPer vector, it says only that a cosmid version of CaSpeR is also available (cosPer). I am afraid that it has never been described any better than that. However, what it amounts to is just the cosPneo with the hsp70-neo gene replaced by the same mini-white gene that is present in CaSpeR. Sorry about this inadequate answer but the details have never been published. Journals do not like to publish papers on such thing these days. And rightly so. But there is no other institutionalized way to make the information accessible and quotable. V. Pirrotta Dept. of Zoology, Sciences 3 University of Geneva 30, quai E. Ansermet CH-1211 Geneva, Switzerland