Subject: cdi insertion, 33rd ADRC
Hello Bev,
I'm curating the 33rd ADRC at the moment and you have an abstract on
page 52 about an enhancer trap lines insertion into cdi. Have you
named the enhancer trap and/or the insertion alleles? Have you
published this data anywhere else?
As I am sure you know we have very little about cdi in the data base at
the moment, this abstract is the only reference, so all you can tell me
will be useful. And, I'm sure I don't really need to tell you this
but, the data you give me will be entered in the database as a personal
communication from yourself as the info is not strictly within the text
of the abstract.
Ele
Subject: Re: cdi insertion, 33rd ADRC
Hi Ele,
> I'm curating the 33rd ADRC at the moment and you have an abstract on
> page 52 about an enhancer trap lines insertion into cdi. Have you
> named the enhancer trap and/or the insertion alleles?
Three different enhancer trap lines were isolated and two (87 and
242), were mapped to the first exon (5' untranslated region) and one
(BA01), to the first intron of cdi. The 242 enhancer trap line and
probably 87 as well have already been cited in other papers. I'm not
sure about BA01. The insertions were all viable and weren't named as
alleles. About 20 lethals were isolated by excising the P element and
their names are not at all informative and they haven't been published.
I guess they'll be renamed 1-20 or wh
> Have you
> published this data anywhere else?
No I haven't. It's been sitting on my CV as 'in preparation' for
years. The lab that I did the work in was trying to carry on (very
slowly) and find a phenotype (other than lethality) for my mutants. As
it turns out, I just found out that the gene was independently found by
another lab as a target gene for Ubx. We sent them my mutants and they
found gut defects in embryos. It's too early to put any of that in
Flybase but hopefully it will be published soon with the new results.
By the way the genes file says the gene name is central divider
while in fact the name we gave it is center divider. Do I need to write
a formal letter to Flybase to correct that?
> As I am sure you know we have very little about cdi in the data base at
> the moment, this abstract is the only reference, so all you can tell me
> will be useful. And, I'm sure I don't really need to tell you this
> but, the data you give me will be entered in the database as a personal
> communication from yourself as the info is not strictly within the text
> of the abstract.
I don't really have that much to add to the record except perhaps
the lethal alleles which were caused by P element excision and were
mapped as small deletions (mostly ~1-3 kb) which take out the
transcription start. 10 of them will appear in the published figure. If
you want to put any of that in I can give you more specific
information. I'm having a hard time finding it at the moment.
Bev
Eleanor.