Strong, R.K., Vaughn, D.E., Bjorkman, P.J., Snow, P.M. (1994). Expression and crystallization of a soluble form of Drosophila fasciclin III. J. Mol. Biol. 241(3): 483--487.
FlyBase ID
FBrf0076869
Publication Type
Research paper
Abstract
A truncated form of Drosophila fasciclin III has been engineered by site-directed mutagenesis. Secreted fasciclin III is expressed at 35 to 40 mg/l in insect cells with baculovirus carrying the recombinant gene. Single crystals of purified soluble fasciclin III have been grown by vapor diffusion versus polyethylene glycol 8000/sodium citrate at low pH. The space group is P6(1)22 or its enantiomorph P6(5)22, with unit cell dimensions a = b = 140 A, c = 260 A. Cryo-preserved crystals diffract to reciprocal lattice spacings beyond 3.0 A.
