Buckingham, S.D., Hosie, A.M., Roush, R.L., Sattelle, D.B. (1994). Actions of agonists and convulsant antagonists on a Drosophila melanogaster GABA receptor, Rdl, homo-oligomer expressed in Xenopus oocytes. Neurosci. Lett. 181(1-2): 137--140.
FlyBase ID
FBrf0078137
Publication Type
Research paper
Abstract
Cytoplasmic injection of Xenopus oocytes with cDNA-derived RNA (cRNA) encoding a wild-type Drosophila melanogaster GABA receptor subunit (Rdl) resulted in functional expression of a GABA receptor homo-oligomer. Membrane currents recorded in response to muscimol, trans (TACA)- and cis (CACA)-4-aminocrotonic acid reversed at membrane potentials close to ECl- and were insensitive to 1.0 x 10(-4) M bicuculline methiodide. An order of potency of GABA approximately muscimol approximately TACA > CACA > glycine was observed. Response of the expressed Drosophila Rdl receptor to GABA was reduced by t-butylbicyclophosphorothionate (TBPS), 4-n-propyl-4'-ethynylbicycloorthobenzoate (EBOB), picrotoxinin and the novel insecticide, fipronil (all at 1.0 x 10(-5) M).
